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| Linard, Clementine; Gueguen, Yannick; Moriceau, Jacques; Soyez, Claude; Hui, Belinda; Raoux, Aurore; Cuif, Jean Pierre; Cochard, Jean-claude; Le Pennec, Marcel; Le Moullac, Gilles. |
| Marine mollusc shell growth has been widely measured using fluorochrome marking. In order to test the efficiency and reliability of calcein staining on P. margaritifera shells and pearls, the present study examined two administration methods, different concentrations and several immersion times.Immersion in a 150 mg.L-1 calcein solution for 12 h to 24 h appeared to be the best method for marking P. margaritifera shells. For pearl marking, injection of a 200 mg.L-1 calcein solution into the pearl pouch was the optimal method. Calcein marking was then used to measure the influence of food resource levels on the shell growth. Groups of 23-month-old P. margaritifera were fed at three trophic levels for two months. The two highest food levels tested (6 000... |
| Tipo: Text |
Palavras-chave: Bivalvia; Pearl; Shell; Growth; Scanning electron microscope. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00028/13907/11195.pdf |
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| Le Moullac, Gilles; Hui, Belinda; Vonau, Vincent; Levy, Peva; Cochard, Jean-claude. |
| Preliminary experiments allowed defining basic requirements of Pinctada margaritifera for somatic and gonadic growth in controlled conditions. This study was continued by observation of the gametogenesis at a cellular level. In April/May 2008, 125 oysters were conditioned for 30 days in 25 tubular tanks (30 L). Water was renewed 4 times/hour. A mixed (v:v) diet of Isochrysis galbana (T-Iso) and Chaetoceros gracilis was supplied continuously. A control batch of 125 oysters was maintained in trays in the lagoon. An initial sample of 30 individuals was made. Gonadic changes were first characterized using a gonadal development index (GDI) based on the ratio of the gonad area to the total visceral mass area on a sagittal section of the body. Histological... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Reproduction; Gametogenesis; Broodstock. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14010/11196.pdf |
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| Hui, Belinda; Demoy-schneider, Marina; Vonau, Vincent; Le Moullac, Gilles; Moriceau, Jacques; Le Pennec, Marcel; Cochard, Jean-claude. |
| Cryopreservation is a useful tool for genetic improvement which has been applied to several bivalve mollusk species. It would allow to keep the gametes of selected individuals and to preserve ex-situ the biodiversity of wild populations of the black-lip pearl oyster Pinctada margaritifera threatened of standardization by the seed transfers between the islands in French Polynesia. The ability to cryopreserve spermatozoa would bring about significant benefits to the cultured black pearl industry. Sperm freezing supposes the control of different steps: preparation of breeders, sperm collection, evaluation of sperm quality and the freezing process itself. Broodstock conditioning in hatchery allows to obtain gametes from the pearl oyster. Sperm quality is... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00029/14043/11238.pdf |
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| Hui, Belinda; Vonau, Vincent; Moriceau, Jacques; Tetumu, Roger; Vanaa, Vincent; Demoy-schneider, Marina; Suquet, Marc; Le Moullac, Gilles. |
| Cryopreservation is a valuable tool for genetic improvement programs. Several bivalve mollusc species have already been the subject of such programs and the Tahitian black pearl oyster industry is now planning the development of selective breeding for desirable traits in Pinctada margaritifera. The ability to cryopreserve spermatozoa would, therefore, offer significant benefits to the cultured black pearl industry. Spermatozoa were cryopreserved with CPA 0.7 M trehalose in 0.8 M Me2SO and a two-step freezing process was used: straws were first maintained in nitrogen vapour for 10 minutes, then directly plunged into liquid nitrogen and stored for one week before use. The viability of thawed sperm was 23 % lower than that of fresh sperm. When using thawed... |
| Tipo: Text |
Palavras-chave: Cryopreservation; Spermatozoa; Fertility; Larval rearing; Pearl oyster; Pinctada margaritifera. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00040/15098/12446.pdf |
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